identification of echinococcus granulosus strains in isolated hydatid cyst specimens from animals by pcr-rflp method in west azerbaijan - iran.
نویسندگان
چکیده
background: the aim of this study was dna extraction from protosco-lecses of echinococcus granulosus and identification of these strains in west- azerbaijan province, north western iran. methods: thirty one livestock isolates from sheep and cattle were collected from abattoirs of the province. to investigate the genetic variation of the isolates, after dna extraction by glass beads-phenol chloroform method; pcr-rlfp analysis of rdna-its1 was performed using three different re-striction enzymes of taq 1, rsa 1 and alu 1 . result: amplified pcr products for all isolates were 1000bp band which is expected band in sheep strains (g1-g3 complex). the results of rflp analy-sis also were the same for all isolates. pcr-rflp patterns restriction en-zymes were identical as follows, rsa1 bands under uv showed two bands approximately 655bp and 345bp. alu 1 bands were as follows: two approx-imately 800bp and 200bp and taq1 did not cut any region and bands were approximately 1000 bp in all samples. conclusions: based on pcr-rflp patterns of its1 fragment produced with endonucleases enzyme digestion in animal isolates, it can be concluded that a single strain of e. granulosus (sheep strain or g1-g3 complex) is domi-nant genotype in this province.
منابع مشابه
Identification of Echinococcus granulosus Strains in Isolated Hydatid Cyst Specimens from Animals by PCR-RFLP Method in West Azerbaijan – Iran
BACKGROUND The aim of this study was DNA extraction from protoscolecses of Echinococcus granulosus and identification of these strains in West-Azerbaijan Province, north western Iran. METHODS Thirty one livestock isolates from sheep and cattle were collected from abattoirs of the province. To investigate the genetic variation of the isolates, after DNA extraction by Glass beads-phenol chlorof...
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BACKGROUND Hydatidosis or cystic hydatid disease is one of the most important diseases in human and animals. Identification of strains is important for improvement of control and prevention of disease. The aim of this study was to determine the strains isolated from human and domestic animals in Ilam Province, Iran, using PCR-RFLP method. METHODS Respectively, 30 and 4 animal and human hydati...
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متن کاملmolecular characterization of echinococcus granulosus from hydatid cysts isolated from human and animals in golestan province, north of iran
b ackground: the aim of the present study was to determine the molecular characteristics of echinococcus granulosus from paraffin-embedded tissues of hydatid cysts isolated from human and protoscoleces of hydatid cysts from sheep, cattle and camel isolates using pcr- rflp of its1- rdna analysis in golestan province, northern iran. m ethods: e . granulosus isolates from human patients infected w...
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عنوان ژورنال:
iranian journal of parasitologyجلد ۸، شماره ۳، صفحات ۳۷۶-۳۸۱
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